The adult Drosophila midgut: A model of human intestinal stem cells and their progeny. The intestines of vertebrates are maintained throughout adult life by the activity of pluripotent intestinal stem cells (ISC's). The molecular pathways that regulate the behaviors of ISC's and their progeny underlie many important human health issues. For example, ISC's or their amplifying progeny are thought to be the source of somatically mutated cells that found new cases of intestinal cancer whereas gastrinomas, vipomas, and carcinoid are all tumors of enteroendocrine cells, a cell type derived from ISC daughters. Enteroendocrine cells produce a large variety of hormones that mediate many of the physiological processes responsible for normal and abnormal intestinal function. Prolonged intravenous nourishment or malabsorption syndromes that can result from Crohn's disease, celiac disease, inflammation or infections result in impaired gut function and may affect the intestinal stem cell itself. Current Research 1. The adult Drosophila midgut is maintained by multipotent stem cells (Ohlstein and Spradling, 2006, 2007). In order to better understand how the behavior of intestinal stem cells and their progeny are regulated, I decided to search for the presence of ISCs in the midguts of adult Drosophila melanogaster, a well studied and well-characterized model organism. The midguts of Drosophila, like vertebrate intestines, are made up of enterocytes interspersed with hormone producing enteroendocrine cells. However, unlike in vertebrates, ISCs had not been previously described in Drosophila. Using lineage labeling, I was able to demonstrate that the Drosophila adult midgut is replenished by a group of intestinal stem cells. Drosophila ISCs, like vertebrate ISCs, are multipotent, producing both enterocytes and enteroendocrine cells. The relatively small cell number and simplicity of the Drosophila midgut allows one to identify ISCs morphologically under various conditions. Moreover, it is possible to remove gene function in marked clones of these cells in order to decipher the nature and directionality of signaling events. Ultimately, a better understanding of the biology of the Drosophila ISC and its progeny, with striking similarities to their vertebrate counterparts, should help with diagnosis, treatment, and eventually cures of a wide spectrum of clinical conditions that affect the human gastrointestinal tract. 2. The adult Drosophila midgut undergoes rapid cell turnover (Ohlstein and Spradling, 2006). The adult Drosophila midgut undergoes rapid cell turnover, like the vertebrate intestine, with turnover virtually complete within one week. The Drosophila midgut is therefore likely to serve as an excellent model system to identify signals that regulate the turnover of epithelial cells in an adult tissue and the mechanism of their removal. Furthermore it provides an excellent opportunity to determine how ISCs respond to changes in cell number that may result from injury, infection, or starvation, to maintain the architecture and integrity of the intestine. 3. Notch signaling regulates ISC differentiation (Ohlstein and Spradling, 2006, 2007). Vertebrate intestines mutant for Notch signaling components produce an excess of secretory cells at the expense of enterocyte production. Significantly, removal of Notch function from Drosophila ISCs also results in the production of excessive enteroendocrine cells, suggesting that the program of enteroendocrine differentiation is likely to be conserved between vertebrates and Drosophila. Interestingly, Drosophila ISCs mutant for either Notch produce an excess of ISCs, a role that has not been addressed in vertebrates. . Future Research 1. Determine the cellular and molecular mechanisms that regulate ISCs in their niche. Work on adult stem cells in many tissues has revealed that many niches often maintain and control stem cell behavior. Defining the existence and nature of a Drosophila intestinal stem cell niche would provide an invaluable model for advancing our understanding of vertebrate ISCs, whose number, location, and regulation within the crypt remain in dispute. 2. Analyze the regulation of gut homeostasis. One of the key products of the ISC is the enteroendocrine population, which constitutes the largest endocrine organ in the body. While enteroendocrine cells represent only 1% of the epithelial cells in the gut, the hormones they produce are involved in such diverse functions as regulating gastric motility, feeding behavior, and utilization of nutrients. The mechanisms controlling the differentiation of these enteroendocrine cells appear to be conserved in Drosophila (Ohlstein and Spradling, 2006, 2007). Therefore, the Drosophila system can be used to gain a much more detailed understanding of the pathways that controls the number, type and function of enteroendocrine. 3. Determine the response of ISCs to changes in diet. Poor nutrition and starvation are known to have detrimental effects on homeostasis in the vertebrate gut. However, the mechanisms at the cellular and molecules that control these responses are currently only poorly known. The ability to manipulate the nutritional environment and genetic background of the Drosophila gut allow powerful new approaches to these questions.